dna oligonucleotides encoding shrna sequences Search Results


dna oligonucleotides encoding shrna sequences  (Biosettia)
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Biosettia dna oligonucleotides encoding shrna sequences
Dna Oligonucleotides Encoding Shrna Sequences, supplied by Biosettia, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ldh-a-derived dna oligonucleotides encoding shrna  (Qiagen)
90
Qiagen ldh-a-derived dna oligonucleotides encoding shrna
Ldh A Derived Dna Oligonucleotides Encoding Shrna, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dna oligonucleotides encoding shrnas  (Eurofins)
90
Eurofins dna oligonucleotides encoding shrnas
Knockdown of Survivin leads to polyploidy in p53-positive tumor cells and isogenic cell lines deficient for p53. HCT116, SBC-2, U87-MG and isogenic HCT116 p53−/− , SBC-2 shp53 , and U87-MG shp53 cells were transduced with <t>shRNAs</t> targeting Survivin (shSurv) or Luciferase (shLuc). a: Representative indirect-immunofluorescence images of transduced SBC-2 cells. The shLuc-transduced cell shows a normal bipolar spindle formation in anaphase. The shSurv-transduced cell shows multipolar spindles and increased numbers of chromosomes. Microtubules (green), membranes (red) and chromosomes (blue). Magnification bars: 10 μm. b: FACS-analyses of cells with knockdown of Survivin depicting the appearance of polyploid cells in wild type and p53-deficient cells. c: Quantitative analysis of the polyploidy phenotype (i.e. cells with <t>DNA</t> contents >4n or >6n for SBC-2) of HCT116, U87-MG and SBC-2 and corresponding p53-negative cells. Data represents mean values and standard error of the mean (SEM). d: Western blot analysis of whole cell lysates demonstrating knockdown of Survivin and accumulation of p53 in Survivin-depleted HCT116, U87-MG and SBC-2 wild type cells but not in controls (shLuc) or p53-deficient cells.
Dna Oligonucleotides Encoding Shrnas, supplied by Eurofins, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
dna oligonucleotides encoding shrnas - by Bioz Stars, 2026-03
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Knockdown of Survivin leads to polyploidy in p53-positive tumor cells and isogenic cell lines deficient for p53. HCT116, SBC-2, U87-MG and isogenic HCT116 p53−/− , SBC-2 shp53 , and U87-MG shp53 cells were transduced with shRNAs targeting Survivin (shSurv) or Luciferase (shLuc). a: Representative indirect-immunofluorescence images of transduced SBC-2 cells. The shLuc-transduced cell shows a normal bipolar spindle formation in anaphase. The shSurv-transduced cell shows multipolar spindles and increased numbers of chromosomes. Microtubules (green), membranes (red) and chromosomes (blue). Magnification bars: 10 μm. b: FACS-analyses of cells with knockdown of Survivin depicting the appearance of polyploid cells in wild type and p53-deficient cells. c: Quantitative analysis of the polyploidy phenotype (i.e. cells with DNA contents >4n or >6n for SBC-2) of HCT116, U87-MG and SBC-2 and corresponding p53-negative cells. Data represents mean values and standard error of the mean (SEM). d: Western blot analysis of whole cell lysates demonstrating knockdown of Survivin and accumulation of p53 in Survivin-depleted HCT116, U87-MG and SBC-2 wild type cells but not in controls (shLuc) or p53-deficient cells.

Journal: Molecular Cancer

Article Title: Survivin safeguards chromosome numbers and protects from aneuploidy independently from p53

doi: 10.1186/1476-4598-13-107

Figure Lengend Snippet: Knockdown of Survivin leads to polyploidy in p53-positive tumor cells and isogenic cell lines deficient for p53. HCT116, SBC-2, U87-MG and isogenic HCT116 p53−/− , SBC-2 shp53 , and U87-MG shp53 cells were transduced with shRNAs targeting Survivin (shSurv) or Luciferase (shLuc). a: Representative indirect-immunofluorescence images of transduced SBC-2 cells. The shLuc-transduced cell shows a normal bipolar spindle formation in anaphase. The shSurv-transduced cell shows multipolar spindles and increased numbers of chromosomes. Microtubules (green), membranes (red) and chromosomes (blue). Magnification bars: 10 μm. b: FACS-analyses of cells with knockdown of Survivin depicting the appearance of polyploid cells in wild type and p53-deficient cells. c: Quantitative analysis of the polyploidy phenotype (i.e. cells with DNA contents >4n or >6n for SBC-2) of HCT116, U87-MG and SBC-2 and corresponding p53-negative cells. Data represents mean values and standard error of the mean (SEM). d: Western blot analysis of whole cell lysates demonstrating knockdown of Survivin and accumulation of p53 in Survivin-depleted HCT116, U87-MG and SBC-2 wild type cells but not in controls (shLuc) or p53-deficient cells.

Article Snippet: All DNA oligonucleotides encoding shRNAs were synthesized with appropriate BamH I and Sal I restriction sites (Eurofins MWG Biotech) to allow ligation into pRVH-1 vectors.

Techniques: Knockdown, Transduction, Luciferase, Immunofluorescence, Western Blot